Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors

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Abstract
Monolayer cultures of human mesothelial cells made quiescent by serum deprivation are induced to undergo one round of DNA synthesis by platelet-derived growth factor (PDGF), epidermal growth factor (EGF), or transforming growth factor type β1 (TGF-β1). This onetime stimulation is independent of other serum components. The kinetics for induction of DNA synthesis observed for PDGF, EGF, and TGF-β1 are all similar to one another, with a peak of DNA synthesis occurring 24-36 h after the addition of the growth factors. Repetitive rounds of DNA synthesis and cell division do not ensue after addition of PDGF, EGF, or TGF-β1 alone or in combination; however, in media supplemented with chemically denatured serum, each of these factors is capable of sustaining continuous replication of mesothelial cells. Stimulation of growth by PDGF and TGF-β1 is unusual for an epithelial cell type, and indicates that mesothelial cells have growth regulatory properties similar to connective tissue cells.— Gabrielson, E. W.; Gerwin, B. I.; Harris, C. C.; Roberts, A. B.; Sporn, M. B.; Lechner, j. F. Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors. FASEB J. 2: 2717-2721; 1988.

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