Molecular heterogeneity of platelet‐activating factor (PAF) in rat glandular stomach determined by gas chromatography/mass spectrometry. PAF molecular species changes upon water‐immersion stress

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Abstract
The molecular heterogeneity of 1‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphocholine (alkylacetyl‐GPC) and 1‐acyl‐2‐acetyl‐sn‐glycero‐3‐phosphocholine (acylacetyl‐GPC) in normal rat glandular stomach was studied by gas chromatography/mass spectrometry (GC/MS) and tandem mass spectrometry. The percentage compositions of the molecular species of 1‐alkyl‐2‐acetyl‐GPC and 1‐acyl‐2‐acetyl‐GPC in the antrum were, respectively. 1‐alkyl [16∶0 (34%) and 18∶0 (66%)]‐2‐acetyl‐GPC and 1‐acyl [16∶0 (60%), 18∶0 (14%) and 18∶1 (26%)]‐2‐acetyl‐GPC. The alkyl chain composition of 1‐alkyl‐2‐acyl‐GPC was quite different from that of 1‐alkyl‐2‐acyl‐GPC in both the antrum and corpus, demonstrating a high degree of selectivity of alkyl chain utilization in PAF biosynthesis. The amount of 1‐acyl‐2‐acetyl‐GPC was much greater than that of 1‐alkyl‐2‐acetyl‐GPC. The molecular heterogeneity of 1‐alkyl‐2‐acetyl‐GPC and 1‐acyl‐2‐acetyl‐GPC in the corpus was similar to that in the antrum. Water‐immersion stress affected not only the amount of 1‐alkyl‐2‐acetyl‐GPC and 1‐acyl‐2‐acetyl‐GPC, but also their molecular heterogeneity in the antrum and corpus. Whereas the amounts of 1‐hexadecyl‐2‐acetyl‐GPC and 1‐acyl [16∶0, 18∶0 and 18∶1]‐2‐acetyl‐GPC decreased markedly (to less than one‐fifth) in the antrum after such stress for 1 hr, the amount of 1‐octadecyl‐2‐acetyl‐GPC increased markedly (up to 4‐fold) in the corpus and severe lesions were observed after stress for 7 hr. The changes may be associated with the pathogenicity of gastric ulcers.

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