Comments on the cytochemical techniques using naphthol AS-BI substrates and hexazonium pararosanilin for the demonstration of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase activities.

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Abstract
After incubation of tissue sections [rat liver and kidney] with naphthol AS-BI substrates and hexazonium pararosaniline methods for ACPase [acid phosphatase], .beta.-Gase [beta glucuronidase], or NAGase [N-acetyl beta glucosaminidase] activity, the following treatment of the sections provides an improved localization and preservation of the azo dye produced as well as a clear nuclear staining rinse sections for 1 min (a half min .times. 2) in distilled water, treat with 70% ethanol for 30 min at room temperature or longer at 4.degree. C, rinse briefly, counterstain with Harris hematoxylin, differentiate in acid alcohol, blue in water, transfer to 80% ethanol for a few minute and mount in 25% polyvinylpyrrolidone-vinyl acetate copolymer in 80% ethanol. For .beta.-Gase, the condition of cold ethanol treatment of sections prior to incubation is modified. A slightly higher activity for NAGase is obtained after preparing the incubation medium by the modified procedure.