Isolation and Characterization of Outermost Layer Deficient Mutant Spores of Bacillus megaterium
- 1 September 1988
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 32 (9) , 973-979
- https://doi.org/10.1111/j.1348-0421.1988.tb01460.x
Abstract
Outermost layer deficient mutant spores of Bacillus megaterium ATCC 12872 were isolated by Urografin density gradient centrifugation after mutagenesis with ethyl methanesulfonate. Although the composition of the cortex peptidoglycan was the same as that of the parent spores, three major proteins (48, 36, and 22 K daltons) were missing, suggesting that these proteins are components of the outermost layer. All mutant spores were also found to have very hydrophobic surface by ‘salt aggregation test,’ which would facilitate selection of such mutants.This publication has 15 references indexed in Scilit:
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