IMMUNOLOGY + CHEMISTRY OF TUBERCULIN .2. CHROMATOGRAPHY WITH SEPHADEX OF NONDIALYZABLE TUBERCULIN-ACTIVE CONSTITUENTS OF BCG CULTURE FILTRATE

1 January 1964

journal article
research article
Published by Elsevier

Vol. 89 (1) , 41-+
https://doi.org/10.1164/arrd.1964.89.1.41

Abstract

Chromatography through Sephadex G-50 columns of the acid-soluble and insoluble, nondialyzable components of BCG culture filtrates resulted in a sharper separation of the protein and polysaccharide constituents. Most of the fractions reacted similarly when tested in guinea pigs sensitized with each of four species of mycobacteria. One carbohydrate-rich fraction displayed a degree of species specificity. Thin film electrophoresis showed that substances with the same precipitating specificities, but with varying electro-phoretic mobilities were present in all fractions which possessed tuberculin reactivity.

Keywords

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IMMUNOLOGY AND CHEMISTRY OF TUBERCULIN .1. ISOLATION OF DIALYZABLE AND NONDIALYZABLE TUBERCULIN-ACTIVE COMPONENTS FROM UNHEATED BCG CULTURE FILTRATES
Published by Elsevier ,1963