Demonstration of the Bacterial Capsule by means of a pH-dependent, Salt-like Combination with Proteins

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Abstract
The capsules of bacteria and of Cryptococcus neoformans can be made visible under the phase-contrast microscope through addition of a number of proteins at a pH range lying on the acid side of the isoelectric point of the proteins. The non-specific capsular reaction is elicited through a salt-like combination of several proteins with the bacterial capsule. The reaction is reversible; it disappears on changing the pH.