Immunofluorescence approach to the study of the ecology of Thermoplasma acidophilum in coal refuse material.
- 1 July 1974
- journal article
- Vol. 28 (1) , 11-6
Abstract
Specific immunofluorescence staining was applied to the study of the localization, distribution, and growth of Thermoplasma acidophilum in its natural habitat, the coal refuse pile. Different antigenic groups of T. acidophilum could be isolated from the same refuse pile, and the same antigenic groups were isolated from piles from different geographical areas. No correlation could be established between the antigenic groups and the pH or temperature of the habitats. Brightly fluorescing cells of T. acidophilum were detected on microscope slides buried in contact with the coal refuse material or immersed in the water in the stream draining a refuse pile. T. acidophilum grew when inoculated into either coal refuse material and/or an aqueous extract of coal refuse when incubated at its optimal temperature of 55 C, but not when incubated at room temperature or 37 C. The coal refuse pile appears to be a primary habitat for T. acidophilum.This publication has 12 references indexed in Scilit:
- Characterization of the membranes of Thermoplasma acidophilum.1973
- Cellular Stability of a Thermophilic, Acidophilic MycoplasmaJournal of General Microbiology, 1972
- Lipids of Thermoplasma acidophilum.1972
- Sulfolobus: A new genus of sulfur-oxidizing bacteria living at low pH and high temperatureArchiv für Mikrobiologie, 1972
- Application of immunofluorescence to studies on the ecology of Sphaerophorus necrophorus.1971
- A Thermophilic, Acidophilic Mycoplasma Isolated from a Coal Refuse PileScience, 1970
- Nonspecific Staining: Its Control in Immunofluorescence Examination of SoilScience, 1968
- Studies with cyanidium caldarium, an anomalously pigmented chlorophyteArchiv für Mikrobiologie, 1959