Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers
- 1 March 1986
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 89 (1-4) , 81-88
- https://doi.org/10.1007/bf01309881
Abstract
In preliminary studies, a system for the propagation of respiratory syncytial virus (RSV) using cytodex 3 microcarriers was established. Optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/L with a cell inoculum density of 4 × 104 cells/ml tissue culture medium. Under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. Maximum yield of virus antigen was achieved after 8 days culture.This publication has 10 references indexed in Scilit:
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