INHIBITORY EFFECT OF 1-BETA-D-ARABINOFURANOSYLTHYMINE 5'-TRIPHOSPHATE AND 1-BETA-D-ARABINOFURANOSYLCYTOSINE 5'-TRIPHOSPHATE ON DNA-POLYMERASES FROM MURINE CELLS AND ONCORNAVIRUS
- 1 January 1978
- journal article
- research article
- Vol. 38 (9) , 3076-3079
Abstract
The effect of the newly synthesized 1-.beta.-D-arabinofuranosyl-thymine 5''-triphosphate was compared with 1-.beta.-D-arabinofuranosylcytosine 5''-triphosphate [an antineoplastic drug] on the activity of DNA polymerases from mouse [myeloma MOPC 104-E] cells and oncornavirus. 1-.beta.-D-Arabinofuranosylthymine 5''-triphosphate inhibited all the activities of DNA polymerase .alpha., .beta. and .gamma. and viral DNA polymerase. The mode of inhibition of 1-.beta.-D-arabinofuranosylthymine 5''-triphosphate as well as 1-.beta.-D-arabinofuranosylcytosine 5''-triphosphate was competitive to the deoxynucleoside triphosphate with the same base. The inhibition constant (K1) and the mode of inhibition of nucleotide incorporation varied with changes in the combination of the inhibitor, substrate(s) and enzyme species.This publication has 8 references indexed in Scilit:
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