Matrix‐assisted laser desorption/ionization time‐of‐flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study

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Abstract
Matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) instrumentation has been used to analyze wheat seed gliadins as an alternative to other established methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), capillary electrophoresis (CE), high‐performance liquid chromatography (HPLC), etc. The MALDI‐TOF approach has shown to have many advantages such as high resolution, cost effectiveness and high throughput. MALDI‐TOF‐based gliadin profiles have been used for fast wheat cultivar identification. However, the genetic information represented by individual gliadin peaks has not been utilized. In this study a wheat doubled haploid population with a genetic linkage map of good coverage was used to assay individual gliadin peaks from MALDI‐TOF profiles as molecular markers. Eight segregating peaks in the population were scored as polymorphic across the population. The 1 to 1 segregating ratios validated the scoring of the peaks and all peaks were mapped to the expected chromosomes or linkage groups on the available linkage map: 1 peak on chromosome 1A, 1 on 6A, 4 on 6B and 2 on 6D. Copyright © 2007 John Wiley & Sons, Ltd.