Passive Cutaneous Anaphylaxis in Mice and Chickens

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Abstract
The sensitivity of the PCA test with different anti-BSA sera was tested in mice. Sensitivity is greatest with isologous or homologous antisera; i.e., as little as 7 x 10-3 [mu]g AbN produced a positive reaction. In contrast, 62 and 250 x 10-3 [mu]g AbN were necessary when rat and rabbit antisera were used, respectively; when chicken anti-BSA was used, the test was negative with as high as 2 [mu]g AbN. The sensitivity of the PCA reaction in mice with isologous and homologous antiserum suggests that it could be a useful tool in screening for circulating antibodies against transplantation antigens. PCA in chickens is described for the first time. Although the sensitivity of this reaction relative to that in mammals is low, it can be elicited with a distantly related antiserum, such as mouse anti-BSA. RPCA in mice was found to depend on the anti-serum-recipient relationship when either BSA or rat whole serum was used as antigen.