Determination of Small Quantities of Nitrogen in Serological Precipitates and Other Biological Materials.

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Abstract
The method described is a modification of the direct-nesslerization procedure of Koch and McMeekin and is suitable for amts. from 5 to 80 [mu]g. The chief innovation is that a given serological precipitate is prepared, washed, digested, and nesslerized in the same 100 mm. x 13 mm. (O.D.) Pyrex tube. For protein solns. suitable vols. are dried in the tubes before analysis. Digestion is carried out with H2O2. A brass digestion rack capable of holding 42 tubes is described. Readings are made with a photoelectric colorimeter. The accuracy is about 98% and the precision of replicate (2 or 3) detns. is [plus or minus] 1-2%. The method is recommended for small samples, costly samples, or large numbers of routine samples.