Detection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay.
- 1 July 1985
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 38 (7) , 733-739
- https://doi.org/10.1136/jcp.38.7.733
Abstract
A simple, sensitive enzyme linked fluorescence immunoassay was developed to detect IgM antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C. trachomatis L2/434 Bu strain were isolated and used as antigens in the assay. Of 113 serum samples obtained from infants with pneumonia, 27 (23.9%) had IgM antibodies to C. trachomatis L2 reticulate bodies and 9 (8.0%) had IgM antibodies to C. trachomatis L2 elementary bodies (titer .gtoreq. 1/500). Specific IgM antibodies were not detected in 20 control serum samples obtained from healthy adults and children. The possible use of enzyme linked fluorescence assay to determine IgM antibodies in the serodiagnosis of C. trachomatis infection is discussed.This publication has 38 references indexed in Scilit:
- Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis.Journal of Clinical Pathology, 1985
- Prevalence of antibodies to Chlamydia trachomatis in Japanese persons determined by microimmunofluorescence using reticulate bodies as single antigenThe Pediatric Infectious Disease Journal, 1984
- Pneumonia due to Chlamydia trachomatis in Japanese infants.The Tohoku Journal of Experimental Medicine, 1984
- Isolation of Chlamydia Trachomatis from Japanese Infancts with PneumoniaPediatrics International, 1983
- Serology of Chlamydia trachomatis in InfantsThe Journal of Infectious Diseases, 1982
- Development and evaluation of an enzyme-linked immunosorbent assay (ELISA), using chlamydial group antigen, to detect antibodies, to Chlamydia trachomatis.Journal of Clinical Pathology, 1982
- Automated measurement of immunogalactosidase reactions with a fluorogenic substrate by the aperture defined microvolume measurement method and its potential application to schistosoma mansoni immunodiagnosisJournal of Immunological Methods, 1980
- Human Serology in Chlamydia trachomatis Infection with MicroimmunofluorescenceThe Journal of Infectious Diseases, 1974
- Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.1972
- Immunoassay using antigen—enzyme conjugatesFEBS Letters, 1971