Isolation of glucosyltransferase fromStreptomyces aureofaciens

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Abstract
Streptomyces aureofaciens B 96 grown on a synthetic medium glucosylated exogenous 1,2-dihydroxy-9, 10-anthraquinone (alizarin). The glucosylation was inhibited by 2,4-dinitrophenol added to the cultivation medium. A cell-free preparation was obtained from the mycelium isolated after 16 h of growth and was found to catalyze the transfer of glucose from glucosyluridyl diphosphate to 1,2-dihydroxy-9, 10-anthraquinone, giving rise to l-hydroxy-2-(β-D-glueopyranosyloxy)-9,10-anthraquinone.