PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION OF TYPES 4 AND 5 ADENOVIRUS-SOLUBLE ANTIGENS

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Abstract
A chromatographic method for the separation and isolation of adenovirus-induced antigens from homogenates of infected cells is described. This procedure, in conjunction with immunological techniques, has been employed to identify, characterize, and compare the soluble antigens of types 4 and 5 adeno-virus-infected HeLa cells. By such means it was shown that adenovirus-infected cells of various origins produce 3 soluble antigens in addition to infectious virus. These include a highly type-specific complement-fixing antigen, a group-specific or common complement-fixing antigen, and a toxin-like material which, when added to normal cells, causes rapid degenerative changes. The immunological behavior of the toxin is that of a group-specific or common adenovirus antigen. It was further shown that similar antigens from types 4 and 5 adenovirus-infected HeLa cells exhibited strikingly different chromatographic characteristics.