Demonstration of the Intracellular Location of Rous Sarcoma Virus Antigen by Fluorescein-Labeled Antiserums

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Abstract
Intracytoplasmic fluorescent material was demonstrated in the cells of the Rous sarcoma by means of fluorescein-labeled anti-Rous sarcoma virus serums. In addition, an occasional cell contained an intranuclear fluorescent body. The number of sarcoma cells containing the fluorescent material was related to the amount of virus inoculated into the chicken to initiate the tumor. The fluorescence was not seen in the cells of normal chicken tissues exposed to the fluorescein-labeled anti-Rous sarcoma virus serums. Prior treatment of the Rous sarcoma tissue, containing virus with unlabeled antiviral serums, blocked the reaction between the intracytoplasmic and intranuclear antigen and the fluorescein-labeled antivirus serums. These findings are interpreted as demonstrating intracytoplasmic and intranuclear virus or viral-associated antigen in cells infected with the Rous sarcoma virus.