Mitochondrial transcription and processing of transcripts during release from glucose repression in ‘resting cells’ of Saccharomyces cerevisiae

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Abstract
Mitochondrial transcription and processing of transcripts was investigated at different stages of release from glucose repression in resting cells of S. cerevisiae. Transcripts were identified by hybridization with nick-translated or terminally labeled gene-specific probes. This allowed the determination of the steady-state levels of individual transcripts in the mitochondrial RNA population. Results showed different gene-specific patterns of response to respiratory induction, such as: no increase in the level of transcripts (oxi2); a rapid increase in the steady-state levels of all transcripts (cob); a very strong increase in the processing of the high-molecular-mass precursors (oxi3 and oli2); an increase in the level of stable circular transcripts (oxi3). As a whole, the results indicate specific and differentiated effects of release from glucose repression on the expression of the different mitochondrial genes, and demonstrate the importance of processing events in mitochondrial regulation.