Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia.
- 1 February 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (3) , 871-875
- https://doi.org/10.1073/pnas.81.3.871
Abstract
A method utilizing reversed-phase high-performance liquid chromatography was developed for the purification to homogeneity of interleukin 2 (IL-2) isolated from a human T-cell leukemia. A final purification of 500,000-fold was obtained with a specific activity of pure IL-2 of 109 units/mg. The amino acid analysis of natural IL-2 is strikingly similar to the composition deduced from sequence analysis of a cDNA coding for human IL-2. Protein sequence analysis of CNBr[cyanogen bromide]-derived peptides yields data consistent with the sequence proposed from cloned cDNA. The availability of homogeneous IL-2 will allow accurate biological studies of its activity free from the contamination of the numerous lymphokine species that are known to be co-produced with IL-2 during the induction procedure.This publication has 36 references indexed in Scilit:
- Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines.The Journal of Immunology, 1980
- Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.The Journal of Experimental Medicine, 1979
- Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines.The Journal of Experimental Medicine, 1979
- Monoclonal cytolytic T-cell lines.The Journal of Experimental Medicine, 1979
- Biochemical Relationship of Thymocyte Mitogenic Factor and Factors Enhancing Humoral and Cell-Mediated Immune ResponsesThe Journal of Immunology, 1978
- T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for ActivityThe Journal of Immunology, 1978
- INVITRO GROWTH OF CYTOTOXIC HUMAN LYMPHOCYTES .1. GROWTH OF CELLS SENSITIZED INVITRO TO ALLOANTIGENS1978
- Long term culture of tumour-specific cytotoxic T cellsNature, 1977
- Selective in Vitro Growth of T Lymphocytes from Normal Human Bone MarrowsScience, 1976
- Amino acid analysis with fluorescamine at the picomole levelArchives of Biochemistry and Biophysics, 1973