Nanofilter array chip for fast gel-free biomolecule separation
- 22 December 2005
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 87 (26) , 263902
- https://doi.org/10.1063/1.2149979
Abstract
We report here a microfabricated nanofilter array chip that can size-fractionate sodium dodecyl sulfate (SDS)-protein complexes and small DNA molecules based on the Ogston sieving mechanism. Nanofilter arrays with a gap size of 40–180 nm were fabricated and characterized. Complete separation of SDS-protein complexes and small DNA molecules were achieved in several minutes with a separation length of 5 mm. The fabrication strategy for the nanofilter array chip allows further increasing of the nanofilter density and decreasing of the nanofilter gap size, leading, in principle, to even faster separation.Keywords
This publication has 24 references indexed in Scilit:
- Two-state migration of DNA in a structured microchannelPhysical Review E, 2005
- The dynamics of genomic-length DNA molecules in 100-nm channelsProceedings of the National Academy of Sciences, 2004
- Continuous Particle Separation Through Deterministic Lateral DisplacementScience, 2004
- Separation of Long DNA Molecules by Quartz Nanopillar Chips under a Direct Current Electric FieldAnalytical Chemistry, 2003
- A DNA prism for high-speed continuous fractionation of large DNA moleculesNature Biotechnology, 2002
- Electrophoretic Separation of Long Polyelectrolytes in Submolecular-Size Constrictions: A Monte Carlo StudyMacromolecules, 2002
- Separation of Long DNA Molecules in a Microfabricated Entropic Trap ArrayScience, 2000
- Electrohydrodynamic Stretching of DNA in Confined EnvironmentsPhysical Review Letters, 1998
- DNA electrophoresis in microlithographic arraysNature, 1992
- The spaces in a uniform random suspension of fibresTransactions of the Faraday Society, 1958