INTRACELLULAR FLUORESCENCE OF PHOTOSENSITIZING PORPHYRINS AT DIFFERENT CONCENTRATIONS OF MITOCHONDRIA

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Abstract
Abstract—Photofrin II, which contains the most efficient components of hematoporphyrin derivative with regard to tumor phototherapy, was measured fluorometrically in single cells of three squamous cell lines with different mitochondrial concentrations. Using the method of time‐resolved fluorescence microscopy, two components—probably monomeric and dimeric species—were differentiated according to the lifetimes of their excited states. The intensity ratio between these components was found to depend on the concentration of mitochondria. This may be due to a monomerization of those porphyrin molecules which are retained in mitochondrial sites.