Evaluation of a two-minute test for urine screening

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Abstract
The ability of a urine filtration system (Bac-T-Screen) to detect negative urine cultures within 2 min was evaluated. Urine specimens (1000) were tested with the Bac-T-Screen and compared with a standard semiquantitative culture plate method and the Autobac system. Of the 1000 clean voided urine specimens tested, 246 specimens had colony counts .gtoreq. 105 [colony-forming units] ml by the culture plate method. Of these, the Bac-T-Screen detected 65.4% (161 of 246); the Autobac detected 63.0% (155 of 246). When pure cultures of diphtheroids, lactobacilli and viridans streptococci other than group D and cultures containing multiple organisms were considered to be contaminants and were therefore excluded, there were 106 pure cultures of probable pathogens of which the Bac-T-Screen detected 76.4% (81 of 106) and the Autobac detected 90.6% (96 of 106). There were 133 specimens uninterpretable with the Bac-T-Screen because 36 clogged the filter and 97 left a residual pigment on the filter. A majority of those clogging the filter (69.4%) had positive plate counts; the majority of the pigmented urines had negative plate counts. Of those urine specimens tested, 754 were negative by the culture plate method. The false-positive rates for Bac-T-Screen and Autobac were 16.2 and 5.8%, respectively. As a urine screen, the Bac-T-Screen has a negative predictive value comparable to the Autobac system and has the advantage of being a 2 min test.

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