Characteristics of human tumour xenografts transplanted under the renal capsule of immunocompetent mice

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Abstract
Human tumor lines established in athymic nude mice were grafted under the renal capsule of immunocompetent mice. Grafts from 27 human tumor lines, comprising 9 malignant melanomas, 10 sarcomas, 2 colon carcinomas, 4 lung carcinomas and 2 mammary carcinomas, grew well under the renal capsule of the immunocompetent mice, and they retained morphological and functional characteristics of the parent tumors, as judged by light microscopy, EM and immunohistochemical examinations. Numerous mitoses were detected. Granulation tissue and necrosis were not predominant features. After day 4, the grafts became infiltrated from the periphery by mouse inflammatory cells. The infiltration could be prevented by pretreatment of the animals with cyclophosphamide. Anti-human antibodies were detected after day 3. Single cell suspensions from the subrenal grafts were able to form colonies in soft agar and upon reimplantation in nude mice. S.c. tumors were formed, showing that the grafted tumor tissue had also retained its malignant character. Altogether, the results support the view that human tumor xenografts grow well under the renal capsule of immunocompetent mice, and the grafts retain important characteristics of the original tumor.

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