The Effect of Temperature on the Nutritional Requirements of Pasteurella pestis

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Abstract
Six strains of P. pestis, both virulent and avirulent, were studied. At 28[degree] C good growth was obtained by adding 5 amino acids to the basal medium containing glucose, haemin, inorganic and ammonium salts. The essential amino acids were phenylalanine, isoleucine, valine, cysteine, and methionine. At 36[degree] 2 additional amino acids[long dash]serine and threonine[long dash]were required for growth, and also biotin and pantothenic acid. Possible reasons for this temp. effect on the nutritional requirements are discussed.