DNA from alkalophilicBacilluscan transformB. subtilisto alkalophily
- 5 April 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 154 (1) , 201-204
- https://doi.org/10.1016/0014-5793(83)80903-x
Abstract
On incubation of B. subtilis RM125(arg15 leuA8 rM− mM−) with DNA from alkalophilic Bacillus, the transformants (Arg+Leu− or Leu−Arg+) appeared at pH 10. The transformants were able to grow even at pH 7. Alkalophilic Bacillus was resistant to bacteriophages π105D1C2·1012 grown on B. subtilis 1012(r-mM+) and π105D1C2·ISMR4 grown on B. subtilis ISMR4rM+rR+mM+mR+), but the recipient B. subtilis and the transformant(Arg+Leu−) were susceptible to both the of the bacteriophages. The results indicate that the transformant is a B. subtilis derivative and that alkalophilicity of alkalophilic Bacillus was transferred to B. subtilisKeywords
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