Use of monoclonal antibodies against dibenzo-p-dioxin degrading Sphingomonas sp. strain RW1
- 1 February 1996
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 22 (2) , 141-144
- https://doi.org/10.1111/j.1472-765x.1996.tb01128.x
Abstract
A monoclonal antibody prepared against surface antigen of Sphingomonas sp. strain RW1 was used for the direct detection of RW1-like organisms in environmental samples by epifluorescence microscopy and subsequent confirmation by Western blot. Of the 76 samples collected from various sources and probed using epifluorescence, only one sample, effluent from paper and pulp processing, gave a positive result. The effluent was cultured and yielded an organism which, by Western blot analysis, was shown to contain the 28 kDa protein recognized by the monoclonal antibody.Keywords
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