Uptake of cell‐penetrating peptides in yeasts
- 2 September 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (23) , 5217-5222
- https://doi.org/10.1016/j.febslet.2005.07.099
Abstract
The uptake of different cell-penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans, was studied using fluorescence HPLC-analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF)3K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studiesKeywords
This publication has 11 references indexed in Scilit:
- Uptake of cell-penetrating peptides is dependent on peptide-to-cell ratio rather than on peptide concentrationBiochimica et Biophysica Acta (BBA) - Biomembranes, 2004
- Delivery of bioactive molecules into the cell: the Trojan horse approachMolecular and Cellular Neuroscience, 2004
- Metabolic cleavage of cell-penetrating peptides in contact with epithelial models: human calcitonin (hCT)-derived peptides, Tat(47–57) and penetratin(43–58)Biochemical Journal, 2004
- Chances and pitfalls of cell penetrating peptides for cellular drug deliveryEuropean Journal of Pharmaceutics and Biopharmaceutics, 2004
- Cell entry and antimicrobial properties of eukaryotic cell‐ penetrating peptidesThe FASEB Journal, 2003
- In vitro Uptake and Stability Study of pVEC and Its All-D AnalogBiological Chemistry, 2003
- Intracellular Concentration Measurements in Adherent Cells: A Comparison of Import Efficiencies of Cell-Permeable PeptidesBiological Chemistry, 2002
- Cargo delivery kinetics of cell-penetrating peptidesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2001
- Cellular uptake of an α-helical amphipathic model peptide with the potential to deliver polar compounds into the cell interior non-endocyticallyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1998
- A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell NucleusJournal of Biological Chemistry, 1997