Crystalline lactic dehydrogenase from heart muscle

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Abstract
The enzyme, which catalyses the reversible reduction of cozymase by lactic acid in animal tissues, was prepared in crystalline form. Starting from the water extract of bullock''s heart tissue, the procedure is adsorption to Ca3(PO4)2; elution with phosphate buffer; precipitation with (NH4)2SO4; precipitation with acetone; fractionation with (NH4)2SO4 between 0.3-0.5 saturation; precipitation with acetone; and crystallization from (NH4)2SO4. Colorless needles result which dissolve readily in water. The pure enzyme is inactivated if dialyzed against distilled water. On the addition of pure heart flavoprotein [see B. A. 13 (9): entry 14780] and cozymase, this crystalline enzyme catalyses the reduction of methylene blue by lactic acid. Lactic and malic dehydrogenases are not identical.

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