Determination of the Fatty Acid Composition of Milk Fat by Dual Column Temperature Programmed Gas-Liquid Chromatography

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Abstract
Methyl-esters were prepared by sealing into a freeze-drying bulb 1 part by weight of milk fat and 2 parts by volume of 0.025 [image] sodium-methylate in methanol. Interesterification was completed by keeping the bulb in a 60[degree]C oven for 1 hr. The contents of the bulb were used for GLC analysis. Temperature programming was from 70-220[degree]C at 7.5[degree]C/min. Correction factors for conversion of peak areas to weight % methyl-ester were determined for saturated acids (from butyric- to stearic-and for oleic-, linoleic- and linolenic-acid).