Immunoglobulin-like surface molecules and theta antigen during the specific and non-specific stimulation of mouse spleen cells in vitro.

  • 1 August 1972
    • journal article
    • Vol. 11  (4) , 523-34
Abstract
BALB/c spleen cells were stimulated with C57B1/6 cells, keyhole limpet haemocyanin (KLH), pokeweed mitogen (PWM) or phytohaemagglutinin (PHA). By the immunofluorescence method the development of transformed cells with immunoglobulin-like receptor molecules or theta antigen was investigated. Transformed Ig-receptor cells were predominant after stimulation with KLH, followed by PWM, PHA and allogeneic cells in decreasing order. Transformed cells with theta-antigen were clearly increased after stimulation with allogeneic cells. With all systems, more transformed cells with Ig-receptors were found in the stimulated cultures than in the control cultures, indicating that all stimulants have an effect on both cell types. The implications of the results on the immunologic interpretation of results obtained by in vitro stimulation of lymphocytes with the different stimulants are discussed.