Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.
- 1 June 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (11) , 3273-3277
- https://doi.org/10.1073/pnas.81.11.3273
Abstract
Plasmids were constructed that direct the synthesis in E. coli of H chains and/or L chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of H chain (Fd'' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of H chain or Fd'' fragment mixed with extracts containing L chain.Keywords
This publication has 33 references indexed in Scilit:
- Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody productionNature, 1983
- Specificities and binding properties of eight monoclonal antibodies against carcinoembryonic antigenMolecular Immunology, 1982
- Increased Synthesis inE. coliof Fibroblast and Leukocyte Interferons Through Alterations in Ribosome Binding SitesDNA, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980
- Cloning and complete nucleotide sequence of mouse immunoglobulin γ1 chain geneCell, 1979
- Separation and purification of the mRNAs for vesciular stomatitis virus NS and M proteinsVirology, 1979
- Direct expression in Escherichia coli of a DNA sequence coding for human growth hormoneNature, 1979
- Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductaseNature, 1978
- DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUESThe Journal of Experimental Medicine, 1965