Selective Killing of Normal and Neoplastic Human B Cells with Anti-CD 19- and Anti-CD22-Ricin A Chain Immunotoxins
- 1 January 1986
- journal article
- research article
- Published by Mary Ann Liebert Inc in Cancer Drug Delivery
- Vol. 3 (4) , 261-272
- https://doi.org/10.1089/cdd.1986.3.261
Abstract
Monoclonal antibodies directed against two human B cell-restricted antigens, CD19 and CD22, were conjugated to highly purified ricin A chain. These A chain immunotoxins (A-IT) were specifically cytotoxic to Daudi cells and normal human peripheral blood B cells in vitro. The concentration required for 50% inhibition of protein synthesis (IC50) in these cells ranged from 7.5 x 10-10 M to 4.2 x 10-9 M. The specific toxicities of these A-ITs for Daudi cells were augmented 2-to 6-fold in the presence of 20mM NH4Cl. These studies demonstrate that A-ITs specific for CD19 and CD22 may be useful in the clinical treatment of B cell malignancies.Keywords
This publication has 24 references indexed in Scilit:
- Use of a novel colony assay to evaluate the cytotoxicity of an immunotoxin containing pokeweed antiviral protein against blast progenitor cells freshly obtained from patients with common B-lineage acute lymphoblastic leukemia.The Journal of Experimental Medicine, 1986
- ANTI-B1 MONOCLONAL ANTIBODY AND COMPLEMENT TREATMENT IN AUTOLOGOUS BONE-MARROW TRANSPLANTATION FOR RELAPSED B-CELL NON-HODGKIN'S LYMPHOMAThe Lancet, 1984
- B cell origin of non-T cell acute lymphoblastic leukemia. A model for discrete stages of neoplastic and normal pre-B cell differentiation.Journal of Clinical Investigation, 1984
- Cytotoxicity of a cell-reactive immunotoxin containing ricin A chain is potentiated by an anti-immunotoxin containing ricin B chain.The Journal of Experimental Medicine, 1984
- In vivo therapy of a murine B cell tumor (BCL1) using antibody-ricin A chain immunotoxins.The Journal of Experimental Medicine, 1982
- Neoplastic B Cells as Targets for Antibody‐Ricin A Chain ImmunotoxinsImmunological Reviews, 1982
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Initial characterization of monoclonal antibodies against human monocytes.Proceedings of the National Academy of Sciences, 1980
- Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.Proceedings of the National Academy of Sciences, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970