Characterisation of stress protein LysU. Enzymic synthesis of diadenosine 5′,5‴-P1,P4-tetraphosphate (Ap4A) analogues by LysU
- 1 January 1996
- journal article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- No. 16,p. 2009-2019
- https://doi.org/10.1039/p19960002009
Abstract
The stress protein LysU (lysyl tRNA synthetase) has been purified from a recombinant strain of Escherichia coli expressing the plasmid pXLys5, and kinetically characterised. Preparative syntheses of analogues of the biologically important molecule diadenosine 5′,5-P1,P4-tetraphosphate (Ap4A) are then achieved in good yield by enzyme catalysis, using purified LysU.Keywords
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