GENETICS OF SOMATIC MAMMALIAN CELLS, X. COMPLEMENTATION ANALYSIS OF GLYCINE-REQUIRING MUTANTS
- 1 December 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (4) , 1284-1291
- https://doi.org/10.1073/pnas.64.4.1284
Abstract
Complementation analysis at the single gene level has been carried out on 13 different, recessive, glycine-requiring mutants produced by action of mutagenic agents on the CHO-K1 Chinese hamster cell. The mutants form four different complementation classes indicating that at least four separate genes can produce a growth requirement for glycine. The four classes of mutants can be distinguished biochemically as well as genetically. Existence of these four mutant classes appears to explain the preponderance of the glycine-requiring forms in the auxotrophs produced by treatment of the parental cell with mutagenic agents.Keywords
This publication has 9 references indexed in Scilit:
- Complementation Analysis on Virus-Fused Chinese Hamster Cells with Nutritional MarkersScience, 1969
- Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.Proceedings of the National Academy of Sciences, 1968
- Studies on serine hydroxymethylase isoenzymes from rat liverBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- INDUCTION OF DIHYDROFOLATE REDUCTASE ACTIVITY BY SV40 AND POLYOMA VIRUS1966
- CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUMProceedings of the National Academy of Sciences, 1965
- Further Studies on Dihydrofolic ReductaseJournal of Biological Chemistry, 1963
- Relation of Folic Acid Reductase to Amethopterin Resistance in Cultured Mammalian CellsJournal of Biological Chemistry, 1961
- The Determination of Formaldehyde and Serine in Biological SystemsPublished by Wiley ,1958
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951