Nucleic Acid Synthesis by Leukocytes in Presence of Anti-Leukocyte Factors.

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Abstract
Normal and pathological human sera were tested for effects on incorporation of nucleic acid precursors into ribonucleic acid and deoxyribonucleic acid during 60-minute incubations. Sera from systemic lupus erythematosus or Felty''s syndrome of rheumatoid arthritis did not differ markedly from normal sera. With serum (compared to glucose-salts control), incorporation of adenine-8-C14 into RNA adenine was higher or unchanged, into RNA guanine lower or unchanged, and into DNA adenine variable; incorporation of thymidine-H3 into DNA was lower. Normal rabbit serum and immune rabbit serum containing agglutinins to human leukocytes were tested for effects on incorporation of precursors into nucleic acids and protein. Immune serum at a concentration causing macroscopic agglutination of the cells gave results similar to those with normal (non-agglutinating) serum.