In vitro Conversion of Thyroxin to Triiodothyronine by Kidney Slices.
- 1 May 1954
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 86 (1) , 137-140
- https://doi.org/10.3181/00379727-86-21031
Abstract
Chromato-graphically pure I131-labeled l-thyroxin was added to surviving kidney slices which were incubated in a modified Krebs-Ringer phosphate solution. Following incubation, the slices were homogenized, extracted with butanol and the thyroxin and triiodothyronine were separated and identified by paper chromatog-raphy. The triiodothyronine was radioactive. This was taken as evidence of deiodination of thyroxin to triiodothyronine. Conversion of thyroxin to triiodothyronine was reduced by excess iodide and abolished by boiling the tissue or by addition of KCN to the media.Keywords
This publication has 7 references indexed in Scilit:
- Single Dimension Chromatographic Separation of Thyroxin and Triiodothyronine.Experimental Biology and Medicine, 1953
- 3:5:3′-Triiodothyronine. 1. Isolation from thyroid gland and synthesisBiochemical Journal, 1953
- 3:5:3′-Triiodothyronine. 2. Physiological activityBiochemical Journal, 1953
- Metabolites of Thyroxine.Experimental Biology and Medicine, 1951
- THE NATURE OF THE CIRCULATING THYROID HORMONE IN GRAVES' DISEASE 1Journal of Clinical Investigation, 1951
- Nature of the Circulating Thyroid HormoneNature, 1949
- THE NATURE OF THE CIRCULATING THYROID HORMONEJournal of Biological Chemistry, 1948