A NEW DIAZIRINE FOR PROTEIN MODIFICATION BY FLASH PHOTOLYSIS: COMPARISON WITH AN AZIDE
- 1 June 1986
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 43 (6) , 601-606
- https://doi.org/10.1111/j.1751-1097.1986.tb05634.x
Abstract
Abstract— A new photolabeling agent, N‐[4–(3‐chlorodiazirin‐3‐yl)benzoyl]glycine (CDBG), a carbene generator, was synthesized. The incorporation of its photolytic products into egg white lysozyme was studied using flash photolysis and compared with incorporation of N‐(4‐azido‐2‐nitro‐phenyl)‐2‐amino ethane sulfonate (NAPT) products into lysozyme and bovine pancreatic ribonuclease A. There was considerable additional incorporation of photolysis products into ribonuclease and lysozyme after termination of flash photolysis when NAPT was used but no additional incorporation when CDBG was used. Protein labeled with NAPT retained the label poorly during electrophoresis in sodium dodecyl sulfate. Lysozyme labeled with CDBG lost little label upon electrophoresis. Neither label was well retained during electrophoresis in 8 M urea. Peptic and tryptic peptides from CDBG labeled lysozyme were differentially labeled.This publication has 18 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Arrangement of subunit IV in beef heart cytochrome c oxidase probed by chemical labeling and protease digestion experimentsBiochemistry, 1983
- Topography of rhodopsin in rod outer segment disk membranes. Photochemical labeling with N-(4-azido-2-nitrophenyl)-2-aminoethylsulfonateBiochemistry, 1980
- Steps in the pathway of the thermal unfolding of ribonuclease A. A nonspecific photochemical surface-labeling studyBiochemistry, 1979
- Study of protein topography with flash photolytically generated nonspecific surface-labeling reagents: surface labeling of ribonuclease ABiochemistry, 1977
- Photochemical labeling of the surface proteins of human erythrocytesBiochemistry, 1974
- Kinetics of the decomposition of diazirinesJournal of the Chemical Society, Perkin Transactions 2, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- A solubilizable acrylamide gel for electrophoresisFEBS Letters, 1970
- Steroid Analogs Lacking Ring C. II. Some Analogs of Progesterone and DesoxycorticosteroneJournal of the American Chemical Society, 1950