Low dose Effect of Ionizing Radiation on Incorporation of Iododeoxyuridine into Bone Marrow Cells

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Abstract
The reduction of the incorporation of IUdR [iododeoxyuridine] in bone marrow cells depended on the time after irradiation and on the microenvironment of the cells. The strongest effect was observed 4 h after irradiation. For absorbed doses above 40 rad, whole-body irradiated mice were more sensitive with respect to depression of IUdR incorporation in bone marrow cells, when the bone marrow cells were labeled in vivo, than in vitro. The converse was observed for very small doses of 1 rad and below. The small doses resulted in a significant depression of IUdR incorporation after in vivo irradiation and in vitro labeling and showed no effect after in vivo irradiation and in vivo labeling. The least effect of radiation was observed after irradiation and labeling in vitro. At the smallest doses the effect apparently was due to functional changes of cellular organelles which control intracellular mechanisms. The reduction of IUdR incorporation at low doses appeared due to functional changes of the cellular membranes.