Production of Colony Stimulating Activity in Mixed Mononuclear Cell Culture

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Abstract
Culture medium was harvested after co-incubation of mononuclear cells collected and pooled from the peripheral blood of 2 different normal [human] donors and was tested for colony-stimulating activity (CSA) in agar culture. With bone marrow from normal donors or peripheral blood from patients with chronic granulocytic leukemia as sources of granulocyte-committed progenitor cells (CRU-c), such mixed mononuclear cell conditioned medium (MMC-CM) showed activity equal to that of unfractionated leukocyte feeder layers and greater than that of CSA prepared from lymphocytes stimulated by phytohemagglutinin. The addition to plates of 2-mercaptoethanol during the preparation of MMC-CM enhanced CSA release. MMC-CM is a convenient source of CSA; its use may be preferable to that of feeder layers when day-to-day reproducibility is essential.