A Simple Technique for Sex Chromatin Analysis in Amniotic Cells of Mouse and Rat Embryos

Abstract
A simple technique has been developed for sex chromatin analysis in the amniotic membrane of rodent embryos. This technique combines the use of 60% acetic acid as a fixative and a carbol fuchsia stain. This technique may be useful for quick sex diagnosis of rodent embryos at midgestation for various purposes in experimental embryology.