Cellular Attachment as a Sensitive Indicator of the Effects of Diagnostic Ultrasound Exposure on Cultured Human Cells

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Abstract
Dispersed cultured human cells (T-1 kidney, FL embryonic kidney, ES and JHA amniotic) were seeded into plastic Petri dishes, incubated for 45 min, exposed to a clinical ultrasound source [total power output, 1.76 .+-. 0.18 (SD) MW] for 0.25-60 min and the medium replaced. Attachment was significantly reduced after only 0.50 min of exposure. For all exposures, sensitivity of the JHA amniotic cells was greater than the kidney lines. Like incremental reduction in attachment, differential sensitivity among cell lines became less pronounced with protracted exposures. Cellular attachment is a sensitive parameter for studying the effects of diagnostic ultrasound.
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