Stabilization of Dry Immobilized Acetylcholinesterase on Nitrocellulose Membrane for Rapid Colorimetric Screening of Its Inhibitors in Water and Biological Fluids.

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Abstract
We show the ability of a gelatin and a BSA-trehalose film to convert normally fragile dry immobilized acetylcholinesterase enzyme (AchE) into a stable reagent on nitrocellulose membrane. The remarkable property of the dry immobilized AchE enzyme preparation is its stability when exposed to temperatures as high as 50°C. The proposed method offers a rapid, simple, and inexpensive means in a non-laboratory setup for qualitative analytical colorimetric screening of AchE inhibitor residues such as pesticides and drugs in water and biological fluids.