Estrogen Formation by the Isolated Perfused Rhesus Monkey Brain

Abstract
Perfusion of two isolated brains from immature male rhesus monkeys with [3H]androstenedione resulted in the identification of free and conjugated [3H]estrone and free [3H]estradiol from the perfusates. In the dissected cerebral tissues, estrogens were recovered only from the hypothalamus and limbic system. The production of estrogens from androstenedione during the 40-minute perfusions in these two experiments totaled 1.58 and 2.83 nanograms.