IDENTIFICATION OF ENZYMES AS GLYCOPROTEINS CONTAINING GLUCOSE OR MANNOSE
Open Access
- 10 September 1974
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 80 (5) , 443-446
- https://doi.org/10.1002/j.2050-0416.1974.tb06793.x
Abstract
One-dimensional affinity electrophoresis and crossed immuno-affinoelectrophoresis with concanavalin A have been applied to show that esterase and acid phosphatase from barley malt are glycoprotein enzymes containing glucose or mannose in their carbohydrate part. The enzymes retained their enzymic activity after binding in macromolecular complexes with concanavalin A. Indications were found of micro-heterogeneity within the carbohydrate moiety of the esterase.Keywords
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