Hybridoma Antibodies Produced against Bromelain Derived Cores of Influenza Virus

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Abstract
Splenic lymphocytes from BALB/c mice immunized with cores of influenza virus, obtained after bromelain cleavage of the surface glycoprotein, were fused with the P3-NS1/1-Ag-1 mouse cell line to yield hybridoma cultures. Among 20 stable cloned hybrid cells secreting monoclonal antibodies, 1 was specific for the nucleoprotein (NP), 11 were specific for the membrane (M) protein and 8 were specific for the hemagglutinin (HA). These cores, used as immunogen, contained only the internal proteins of the influenza virus, namely the 3 polymerases, the NP and the M protein and no HA when examined by standard procedures of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and hemagglutination activity. It thus appeared that a small amount of contaminating antigens can sensitize a sufficient number of mouse B cells to be selected as partners. These antibodies were provisionally assigned as anti-carbohydrate attached to the HA.