THE EFFECT OF ULTRAVIOLET-IRRADIATION ON THE LOCALIZATION OF SYNGENEIC AND ALLOGENEIC LYMPHOID-CELLS AND ON ALLOIMMUNOGENICITY

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 44  (2) , 281-287
Abstract
UV irradiation was found to have only a modest inhibitory effect on the alloimmunogenicity of murine lymphoid cells in vivo when the response was assessed by the primary cytotoxic antibody response. UV irradiation had no effect on the initial (1 h) organ localization of Cr-labeled lymphoid cells in syngeneic or allogeneic recipients using either CBA or DBA/2 cells. The 24 h localization in peripheral lymph nodes was considerably and similarly depressed in syngeneic and allogeneic recipients. This effect was shown, by alloantibody treatment of recipients of allogeneic cells, to be due mainly to lack of entry into lymph nodes after 1 h. In agreement with much of the published data, control data for these experiments indicated little difference in lymph node localization of lymph node cells in allogeneic and syngeneic recipients using both CBA and DBA/2 cells in both CBA and DBA/2 recipients. The localization of spleen cells in lymph nodes was found to be rather more sensitive to the allogeneic environment though not as much as has been previously described for spleen cells in contrast to the long established data on lymph node cells. A marked difference in the degree of lymph node localization of CBA and DBA/2 cells was found regardless of the recipient strain.