Some Sources of Variability in Cultures of Entozoic Amoebae. I. The Effect of Age of Culture, Size of Inoculum and Amount of Starch

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Abstract
In factorial expts. on a strain of Entamoeba terrapinae in monobacterial association with an atypical strain of Escherichia coli the major sources of excess variation were associated with age of culture and the amount of rice starch. Deviations associated with size of inoculum were not significantly greater than those due to all other factors. The observed variation was found to be associated with the use of chronologically different pooled cultures as inoculums for the separate segments of the study. Components of ground rice other than starch were shown to be ineffective as growth stimulants.