LOCALIZATION OF GLUCOSE, GLUCONATE, AND GLUCOSE-6-PHOSPHATE OXIDATION SYSTEMS IN EXTRACTS OF PSEUDOMONAS FLUORESCENS
- 1 February 1958
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 4 (1) , 1-7
- https://doi.org/10.1139/m58-001
Abstract
Cell-free extracts of Pseudomonas fluorescens, strain OSU 64, derived by means of sonic oscillation and alumina grinding, were assayed to determine the localization of enzymes necessary for the oxidation of glucose, gluconate, and glucose-6-phosphate. Glucose and gluconate were oxidized to 2-ketogluconate by both the supernatant and particulate fractions of the extracts. Approximately 40% of the activity for the oxidation of glucose was demonstrated to be in the supernatant fraction and 60% in the particulate fraction. Activity for the oxidation of gluconate was found to be located with approximately 50% in each fraction. Oxidation of glucose-6-phosphate was observed only in the soluble portion.Keywords
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