Separation of B and C Type Virions by Centrifugation in Gentle Density Gradients
- 1 May 1974
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 13 (5) , 1143-7
- https://doi.org/10.1128/jvi.13.5.1143-1147.1974
Abstract
Murine type B particles were separated from type C (Rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. The best separation was obtained when the density ranged from 1.13 to 1.20 g/cm 3 when sucrose was used and from 1.12 to 1.28 g/cm 3 with CsCl. The buoyant densities of the B and C particle bands in sucrose were 1.18 and 1.16 g/cm 3 , respectively. The CsCl gradient gave a better separation with the B particles banding at a density of 1.20 g/cm 3 and with the C particle density little different from its value in sucrose.Keywords
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