FLUORESCENT AND HISTOCHEMICAL REACTIONS IN THE RAT THYROID GLAND AT DIFFERENT STATES OF PHYSIOLOGICAL ACTIVITY11,

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Abstract
A typically blue fluorescence of the thyroid colloid appeared associated with its thyroglobulin content. I-poor colloid failed to show the characteristic color. Connective tissue stroma and thyroid follicles were also fluorescent. Birefringent elements were observed in the follicle cells and in the stromal tissues. Mineral salts, as detd. by ash conc. studies, were decreased in hyperplastic glands. Peroxidase and cytochrome oxidase were demonstrated in the follicular cells. The peroxidase reaction was easily inhibited by addition of thiouracil to the reagents, although the cytochrome oxidase reaction was unaffected by such treatment. The observations bear upon the mode of formation of thyroxin and suggest a possible action of antithyroid drugs in preventing the synthesis of the thyroid hormone.