Molecular cloning and characterization of a gibberellin-inducible, putative ?-glucosidase gene from barley
- 1 January 1996
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 30 (2) , 229-241
- https://doi.org/10.1007/bf00020110
Abstract
A putative α-glucosidase clone has been isolated from a cDNA library constructed from mRNA of barley aleurones treated with gibberellin A3 (GA). The clone is 2752 bp in length and has an uninterrupted open reading frame encoding a polypeptide of 877 amino acids. A 680 amino acid region is 43% identical to human lysosomal α-glucosidase and other glycosyl hydrolases. In isolated aleurones, the levels of the corresponding mRNA increase strongly after the application of GA, similar to the pattern exhibited by low-pI α-amylase mRNA. High levels are also observed in the aleurone and scutellum after germination, while low levels are found in developing seeds. The genome contains a single form of this α-glucosidase gene and two additional sequences that may be related genes or pseudogenes.Keywords
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